Journal: bioRxiv

Article Title: Alternative polyadenylation drives isoform-dependent m⁶A remodeling during Zika virus infection

doi: 10.64898/2026.03.27.714597

Figure Lengend Snippet: A) Experimental workflow of GLORI-seq protocol to identify differential m 6 A following ZIKV infection (MOI 1, 48 hpi, n=3). B) Scatter plot showing ZIKV-induced changes in m 6 A levels of individual sites. Orange and blue dots represent upregulated (n=2004) and downregulated (n=502) m 6 A sites during ZIKV infection, respectively. C) Representative gene track plots of upregulated m 6 A in RIOK3 (upper panel), downregulated m 6 A in CIRBP (middle panel), and unchanged m 6 A in ACTB (lower panel). D) Metagene profiles showing the distributions of upregulated, downregulated, and unchanged m 6 A sites across the mRNA molecule. E) The top 6 motifs across upregulated, downregulated, and unchanged m 6 A sites and the proportion of m 6 A sites they contain. F) The absolute difference in m 6 A level of upregulated and downregulated m 6 A sites across the top 6 motifs. G) PANTHER GO enrichment analysis for genes with upregulated m 6 A sites using a binomial test with FDR correction (FDR p < 0.05).

Article Snippet: 2.5 μg of HCV PAMP RNA was transfected into cells for 8 h using the Mirus mRNA transfection kit.

Techniques: Infection

Journal: bioRxiv

Article Title: Alternative polyadenylation drives isoform-dependent m⁶A remodeling during Zika virus infection

doi: 10.64898/2026.03.27.714597

Figure Lengend Snippet: A) and B) The correlation of m 6 A levels for sites detected by GLORI-seq between three biologically independent samples of mock- (A) or ZIKV-infected (B) Huh7 cells (48 hpi, MOI 1). C) Boxplots showing distribution of methylation level of m 6 A sites in Mock and ZIKV conditions. D) Principal component analysis of GLORI-seq m 6 A stoichiometry rates for Mock and ZIKV conditions. E) Metagene profiles showing the distributions of Mock and ZIKV m 6 A sites across the mRNA molecule. F) The top 6 motifs across Mock and ZIKV m 6 A sites and the proportion of m 6 A sites they contain.

Article Snippet: 2.5 μg of HCV PAMP RNA was transfected into cells for 8 h using the Mirus mRNA transfection kit.

Techniques: Infection, Methylation

Journal: bioRxiv

Article Title: Alternative polyadenylation drives isoform-dependent m⁶A remodeling during Zika virus infection

doi: 10.64898/2026.03.27.714597

Figure Lengend Snippet: A) and B) RT-qPCR analysis (relative to RPL30 ) of RNA expression of ALCAM-S and ALCAM-L (A) and NHSL1-S and NHSL1-L (B) upon DENV infection (48 hpi, MOI 1). C) and D) RT-qPCR analysis (relative to RPL30 ) of RNA expression of ALCAM-S and ALCAM-L (C) and NHSL1-S and NHSL1-L (D) upon WNV infection (48 hpi, MOI 0.01). E) and F) RT-qPCR analysis (relative to RPL30) of RNA expression of ALCAM-S and ALCAM-L (E) and NHSL1-S and NHSL1-L (F) upon HCV infection (48 hpi, MOI 1). G) and H) RT-qPCR analysis (relative to RPL30 ) of RNA expression of ALCAM-S and ALCAM-L (G) and NHSL1-S and NHSL1-L (H) upon transfection with HCV PAMP (8 hrs).

Article Snippet: 2.5 μg of HCV PAMP RNA was transfected into cells for 8 h using the Mirus mRNA transfection kit.

Techniques: Quantitative RT-PCR, RNA Expression, Infection, Transfection

Journal: bioRxiv

Article Title: Regulation and Function of the HPV16 CircE7 RNA

doi: 10.64898/2026.03.09.710444

Figure Lengend Snippet: (A) RT-qPCR of circE7 and E6*I normalized to linear E6 RNA from primary keratinocytes transfected with HPV16 WT or HPV16 mut2 genomes (circE7 splicing acceptor m 6 A motif mutation, no change to AA sequence). *p<0.05, ****p<0.0001. (B) Western blot of p53, GAPDH and HSP90 in whole cell extracts from Keratinocytes transfected with HPV16 WT or mut2 genomes. (C) Viral DNA detected by qPCR after HIRT DNA extraction out of primary Keratinocytes transfected with either wild type or mut2 HPV16. ****p<0.0001. (D) Crystal Violet staining of keratinocytes grown in 10% BCS DMEM for 2-6 weeks. Cells were transfected with either wild type HPV16 or HPV16 Mut2. Representative images (left) and quantification at week 6 (right). ***p<0.001

Article Snippet: Briefly, early passage primary keratinocytes were co-transfected with an equal DNA ratio of either pHPV16ANsL WT or Mut2 and pCAGGS-nlsCre plasmids (gift from CM Chiang lab) using TransIT Keratinocyte Transfection Reagent (MirusBio, MIR 2800) according to the manufacturer’s protocol.

Techniques: Quantitative RT-PCR, Transfection, Mutagenesis, Sequencing, Western Blot, DNA Extraction, Staining